Virological, Molecular And Immuno-Biochemical Studies Of Lumpy Skin Disease In Naturally Infected Cattle

This study aimed to diagnose Lumpy skin desease by isolation and identification of LSD from suspected naturally infected cattle its effect on immuno-biochemical parameters, inflammatory oxidative stress markers. The was conducted 25 cattle, 1-3 years age two cities in the Sharkia governorate. Ten clinically healthy represented control group fifteen diseased cases group. clinical signs showed anorexia, nodules all over body, fever above 40˚C, edema forelimbs dewlap, enlarged superficial lymph nodes, retarded milk production, abortion some cases. virological examination LSDV samples done. Isolation done MDBK cell line embryonated chiken egg through chorioallantoic membrane route followed using fluorescence technique . Molecular studies including phylogenetic analysis for three positive isolates  similarity previously locally isolated strains Egypt close relation SPPV. Immuno-biochemical results revealed a significant increase aspartate aminotransferase , alanine alkaline phosphatase Malondialdehyde Tumor necrosis factor Interleukin4,immunoglobulin M  immunoglobulin G Meanwhile, decrease total protein, albumin, globulin, Glutathione peroxidase, phagocytic assay, calcium, inorganic phosphorous, sodium, potassium with non-significant change magnesium seen when compared ones. It could be concluded that infection LSD  induce severe  effects immuno-biochemical, antioxidant markers causing great economic loss